酵素化学研究室

2021年度

Comparison of sensitivity and rapidness of PCR, recombinase polymerase amplification, and RNA-specific amplification for detection of Rice yellow mottle virus.
Juma KM, Kojima K, Takita T, Natsuaki K, and Yasukawa K (2021) J Biol Macromol, 21:27-32

Insight into the mechanism of thermostabilization of GH10 xylanase from Bacillus sp. strain TAR-1 by the mutation of S92 to E.
Suzuki M, Takita T, Kuwata K, Nakatani K, Li T, Katano Y, Mizutani K, Mikami B, Yatsunami R, Nakamura S, and Yasukawa K (2021) Biosci Biotechnol Biochem, 85:386-390

Solvent engineering studies on recombinase polymerase amplification.
Kojima K, Juma KM, Akagi S, Hayashi K, Takita T, O'Sullivan CK, Fujiwara S, Nakura Y, Yanagihara I, and Yasukawa K（2021） J Biosci Bioneng, 131:219-224

Insight into the collagen-degrading activity of a serine protease in the latex of Ficus carica cultivar Masui Dauphine.
Nishimura K, Higashiya K, Ueshima N, Kojima K, Takita T, Abe T, Takahashi T, and Yasukawa K (2021) Biosci Biotechnol Biochem, 85:1147-1156

Improvement of Moloney murine leukemia virus reverse trascriptase thermostability by introducing a disulfide bridge in the ribonuclease H region.
Narukawa Y, Kandabashi M, Li T, Baba M, Hara H, Kojima K, Iida K, Hiyama T, Yokoe S, Yamasaki T, Takita T, and Yasukawa K (2021) Protein Eng Des Sel, 34:gzab006

シマグワ茶パウダーを配合したパンの血糖値上昇抑制効果
久米大祐、喬穎、中山珠里、保川清、島尻佳典、伊東昌章（2021）日本栄養・食糧学会誌 74/1:15-20

Development of an efficient one-step real-time Reverse transcription polymerase chain reaction method for severe acute respiratory syndrome-coronavirus-2 detection.
Nakura Y, Wu HN, Okamoto Y, Takeuchi M, Suzuki K, Tamura Y, Oba Y, Nishiumi F, Hatori N, Fujiwara S, Yasukawa K, Ida S, and Yanagihara I (2021) PLoS One, 16:e0252789

Inhibitory effect of Morus australis leaf extract and its component iminosugars on intestinal carbohydrate-digesting enzymes.
Qiao Y, Ito M, Kimura T, Ikeuchi T, Takita T, and Yasukawa K (2021) J Biosci Bioeng, in press

The mutation of Thr315 to Asn of GH10 xylanase XynR increases the alkaliphily but decreases the alkaline resistance.
Kuwata K, Suzuki M, Takita T, Yatsunami R, Nakamura S, and Yasukawa K (2021) Biosci Biotechnol Biochem, 85:1853-1860

Optimization of reaction condition of recombinase polymerase amplification to detect SARS-Cov-2 DNA and RNA using a statistical method.
Juma KM, Takita T, Yamagata M, Ito K, Arikawa E, Akagi S, Kojima K, Biyani M, Fujiwara S, Nakura Y, Yanagihara I, and Yasukawa K (2021) Biochem Biophys Res Commun, 567:195-200

Deveproment of robust isothermal RNA amplification assay for lab-free and lab-quality testing of RNA viruses.
Biyani R, Kirti S, Kojima K, Biyani M, Sharmad V, Kumawatd T, Juma KM, Yanagihara I, Fujiwara S, Kodama E, Takamura Y, Takagi M, Yasukawa K, and Biyani M (2021) Sci Rep, 11:15997

クワ葉成分による糖質分解酵素の阻害と食後高血糖の抑制
保川清、喬穎、伊東昌章、久米大祐 (2021) 食品・臨床栄養　e2021:11-18